Keith Weninger Group

Single Molecule Biophysics at North Carolina State University

Physics Department




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Single molecule FRET

Membrane Fusion

Energy is transferred from one dye to the other When two vesicles fuse their contents mix and the dye dequenches

Recent Publications


V.C. DeRocco, L.E. Sass, R. Qiu, K.R. Weninger, D.A. Erie "Dynamics of MutS-mismatched DNA complexes are predictive of their repair pheontypes", Biochemistry,53, 2043-2052 (2014) link or pubmed

S.M. Mooney, R. Qiu, J.J. Kim, E.J. Sacho, K. Rajagopalan, D. Johng, T. Shiraishi, P. Kulkarni, K.R. Weninger "The Cancer/Testis antigen PAGE4, a regulator of c-Jun transactivation, is phosphorylated by Homeo-domain interacting protein kinase 1, a component of the stress-response pathway", Biochemistry,53, 1670-1679 (2014) link or pubmed or reprint

K. Rajagopalan, R. Qiu, S.M. Mooney, S. Rao, T. Shiraishi, E. Sacho, H. Huang, E. Shapiro, K.R. Weninger, P. Kulkarni "The Stress-response protein prostate-associated gene 4, interacts with c-Jun and potentiates its transactivation", Biochim Biophys Acta.,1842, 154 (2014) link or pubmed


A.T. Brunger, P. Strop, M. Vrljic, M. Bowen, S. Chu, K.R. Weninger "Macromolecular Models by single-molecule FRET", J.D. Puglisi and M.V. Margaris (eds.), Biophysics and Structure to Counter Threats and Challenges, NATO Science for Peace and Security Series B: Physics and Biophysics, 1-19 (2013) ( DOI 10.1007/978-94-007-4923-8 1) link or pdf


U.B. Choi, K.R. Weninger, M.E. Bowen "Immobilization of proteins for single-molecule fluorescence resonance energy transfer measurements of conformation and dynamics", Methods Mol Bio, 896, 3-20 (2012) link or try Pubmed

R. Qiu, V.C. DeRocco, C. Harris, A. Sharma, M.M. Hingorani, D.A. Erie, K.R. Weninger "Large conformational changes in MutS during DNA scanning, mismatch recognition and repair signalling", The EMBO Journal, 31, 2528-2540 (2012) link

S. Khalid, B. Kappus, K. Weninger, S. Putterman "Opacity and transport measurements reveal that dilute plasma models of sonoluminescence are not valid", Physical Review Letters, 108, 104302 (2012) link or see also Physics Today


K. R. Weninger, "Complexin arrests a neighbor", Nature Structural and Molecular Biology, 18, 861 (2011) link

U.B. Choi, J.J. McCann, K. Weninger, M. Bowen, "Beyond the random coil: Stochastic conformational switching in intrinsically disordered proteins", Structure, 19, 566 (2011) link

A.T. Brunger, P. Strop, M. Vrljic, S. Chu, K. Weninger, "Three-dimensional molecular modeling with single molecule FRET", J. Struct. Biol., 173,497-505 (2011) link


V.C. DeRocco, T. Anderson, J. Piehler, D.A. Erie, K. Weninger, "Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes", BioTechniques, 49, 807 (2010) link

J.J. McCann, U.B. Choi, L. Zheng, K. Weninger, M.E. Bowen, "Optimizing methods to recover absolute FRET efficiency from immobilized single molecules", Biophysical Journal, 99, 961 (2010) link

L.E. Sass, C. Lanyi, K. Weninger, D.A. Erie, "Single-molecule FRET TACKLE reveals highly dynamic mismatched DNA-MutS complexes", Biochemistry, 49, 3174 (2010) link

U.B. Choi, P. Strop, M. Vrljic, S. Chu, A. T. Brunger, K. Weninger, "Single molecule FRET derived model of the synaptotagmin 1 - SNARE fusion complex", Nature Structural and Molecular Biology, 17, 318 (2010) link

J. Sakon, K. Weninger, "Detecting the conformation of individual proteins in live cells", Nature Methods, 7, 203 (2010) link

P. Fletcher, M. Fletcher, K. Weninger, T. Anderson, B. Martin "VAMP cleavage by a new metalloprotease from the Brazilian scorpion Tityus serrulatus", J. Biol Chem., 285, 7405 (2010) link


K. Weninger, "How do adhesion proteins stick?", Structure, 17, 1035 (2009) link

L.Wessels, K. Weninger, "Physical aspects of viral membrane fusion", TheScientificWorldJOURNAL, 9, 764 (2009) link

A. Brunger, K. Weninger, M. Bowen, S. Chu "Single-molecule studies of the neuronal SNARE fusion machinery", Annual Review of Biochemistry, 78, 903 (2009) link

W. Thongthai, K. Weninger, "Photoinactivation of Sindbis virus infectivity without inhibition of membrane fusion", Photochemistry and Photobiology, 85, 801 (2009) link

J.J. Sakon, G.J. Ribeill, J.M. Garguilo, J. Perkins, K.R. Weninger, R.J. Nemanich, "Fluorescence quenching effects of nanocrystalline diamond surfaces", Diamond and Related Materials, 18, 82 (2009) link


K. Weninger, M. Bowen, U.B. Choi, A. Brunger, S. Chu, "Accessory proteins stabilize the acceptor complex for synaptobrevin, the 1:1 syntaxin/SNAP-25 complex", Structure, 16, 308 (2008) link or pdf


L. Wessels, M.W. Elting, D. Scimeca, K. Weninger, "Rapid membrane fusion of individual virus particles w/supported lipid bilayers", Biophysical Journal, 93, 526 (2007) link or pdf

Y. Li, G. Augustine, K. Weninger, "Kinetics of complexin binding to the SNARE complex: correcting single molecule FRET measurements for hidden events", Biophysical Journal, 93, 2178 (2007) link or pdf

G. Wang, R. Hernandez, K. Weninger, D.T. Brown "Infection of cells by Sindbis virus at low temperature", Virology, 362, 461 (2007) link or pdf


M. Bowen, K. Weninger, J. Ernst, S. Chu, A. Brunger, "Single-molecule studies of synaptotagmin and complexin binding to the SNARE complex", Biophysical Journal, 89, 690 (2005) link or pdf


M. Bowen, K. Weninger, A. Brunger, S. Chu, "Single molecule observation of liposome - bilayer fusion thermally induced by SNAREs", Biophysical Journal, 87, 3569 (2004) link or pdf


K. Weninger, M. Bowen, S. Chu, A. Brunger, "Single-molecule studies of SNARE complex assembly reveals parallel and antiparallel configurations", PNAS, 100, 14800 (2003) link or pdf

A publication from my older work

S. Putterman, K. Weninger, "Sonoluminescence: How bubbles turn sound into light", Annual Review of Fluid Mechanics, 32, 45 (2000) link or pdf

Complete List of Publications including older papers